Group II phospholipase A 2 induced by interleukin-1β in cultured rat gingival fibroblasts

Previously, we reported the presence of group II-like phospholipase A 2 activity in the soluble fraction of rat gingiva. In the present study, we found that treatment of rat gingival cells with human recombinant interleukin- 1β resulted in dose-dependent stimulation of intracellular and extracellula...

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Bibliographic Details
Published in:FEBS letters 1992-06, Vol.304 (1), p.69-72
Main Authors: Shinohara, Hiroyuki, Amabe, Yukari, Komatsubara, Takanori, Tojo, Hiromasa, Okamoto, Mitsuhiro, Wakano, Yoichi, Ishida, Hiroshi
Format: Article
Language:English
Subjects:
Interleukin-1β
Periodontitis
Phospholipase A 2
Rat gingival fibroblast
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Summary:Previously, we reported the presence of group II-like phospholipase A 2 activity in the soluble fraction of rat gingiva. In the present study, we found that treatment of rat gingival cells with human recombinant interleukin- 1β resulted in dose-dependent stimulation of intracellular and extracellular phospholipase A 2 activity. Antisera against group II phospholipase A 2 totally blocked the interleukin-1β-induced phospholipase A 2 activity, but antisera against group I phospholipase A 2 did not. Moreover, immunoblot analysis showed that the induced phospholipase A 2 was group II phospholipase A 2. These findings suggest that the induced enzyme belongs to the group II phospholipase A 2 family of proinflammatory enzymes.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)80591-4