Ca 2+/calmodulin-activated protein phosphatase : (PP2B) of Saccharomyces cerevisiae PP2B activity is not essential for growth

Protein phosphatase (PP2B) whose activity is stimulated 12-20-fold by Ca 2+/calmodulin (CaM) was partially purified by CaM-Sepharose and heparin-agarose chromatographies from cell extract of the yeast Saccharomyces cerevisiae. PP2B activity was not detectable in a mutant in which two genes ( CMP1 an...

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Bibliographic Details
Published in:FEBS letters 1992-08, Vol.309 (1), p.103-106
Main Authors: Nakamura, Taro, Tsutsumi, Hiroko, Mukai, Hideyuki, Kuno, Takayoshi, Miyakawa, Tokichi
Format: Article
Language:English
Subjects:
Gene disruptions
Protein phosphatase type 2B
Saccharomyces cerevisiae
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Summary:Protein phosphatase (PP2B) whose activity is stimulated 12-20-fold by Ca 2+/calmodulin (CaM) was partially purified by CaM-Sepharose and heparin-agarose chromatographies from cell extract of the yeast Saccharomyces cerevisiae. PP2B activity was not detectable in a mutant in which two genes ( CMP1 and CMP2) encoding homologs of mammalian PP2B catalytic subunit were disrupted. We have previously shown that the double gene disruption has no significant effect on the growth of yeast [1991, Mol. Gen. Genet. 227, 52–59]. The results indicated that CMP1 and CMP2 are the only genes that encode the PP2B catalytic polypeptide in S. cerevisiae,and PP2B activity is not essential for the growth of the yeast under normal conditions.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)80749-7