Noradrenaline release from permeabilized synaptosomes is inhibited by the light chain of tetanus toxin

Noradrenaline release from rat brain cortical synaptosomes permeabilized with streptolysin O can be triggered by μM concentrations of free Ca 2+. This process was inhibited within minutes by tetanus toxin and its isolated light chain, but not by its heavy chain. The data demonstrate that the effect...

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Bibliographic Details
Published in:FEBS letters 1992-11, Vol.312 (2), p.192-194
Main Authors: Stecher, Brigitte, Hens, Jacques J.H., Weller, Ulrich, Gratzl, Manfred, Gispen, Willem-Hendrik, De Graan, Pierre N.E.
Format: Article
Language:English
Subjects:
Animals
Bacteriology
Biological and medical sciences
BoNT/A
botulinum A toxin
Cell Membrane Permeability - drug effects
Exocytosis
Fundamental and applied biological sciences. Psychology
heavy chain
light chain
Male
Microbiology
noradrenaline
Norepinephrine - metabolism
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Permeabilization
Rats
Rats, Wistar
SLO
Streptolysin O
Synaptosome
Synaptosomes - drug effects
Synaptosomes - metabolism
Tetanus toxin
Tetanus Toxin - pharmacology
TeTx
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Summary:Noradrenaline release from rat brain cortical synaptosomes permeabilized with streptolysin O can be triggered by μM concentrations of free Ca 2+. This process was inhibited within minutes by tetanus toxin and its isolated light chain, but not by its heavy chain. The data demonstrate that the effect of tetanus toxin on NA release from purified synaptosomes is caused by the intraterminal action of its light chain.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)80933-8