Effect of perturbing diacylglycerol metabolism on cytosolic free Ca 2+ oscillations induced in single hepatocytes

Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca 2+-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC 8) inhibit free Ca oscillations induced by phenylephrine and v...

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Bibliographic Details
Published in:FEBS letters 1992-11, Vol.312 (1), p.57-60
Main Authors: Sanchez-Bueno, Antonio, Cobbold, Peter H.
Format: Article
Language:English
Subjects:
Ca 2+ oscillation
Diacylglycerol
diC 8
R 59 022
Single hepatocyte
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Summary:Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca 2+-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC 8) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These results agree with a negative feedback effect of protein kinase C on free Ca oscillations.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)81409-F