Cloning and expression of a cDNA for the rat k-opioid receptor

We cloned a cDNA for the rat K-opioid receptor from a rat thalamus cDNA library. The deduced amino acid sequence consists of 380 residues with features shared by members of the G protein-coupled receptor family. The specific binding of [ 3H]bremazocine to the membrane of COS-7 cells transfected with...

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Bibliographic Details
Published in:FEBS letters 1993-08, Vol.329 (3), p.291-295
Main Authors: Minami, Masabumi, Toya, Takashi, Katao, Yoshikazu, Maekawa, Keiko, Nakamura, Shunya, Onogi, Tatsuhiro, Kaneko, Shuji, Satoh, Masamichi
Format: Article
Language:English
Subjects:
DAMGO, [d-Ala,MePhe4,Gly(ol)5]enkephalin
DPDPE, [d-Pen2 d-Pen5enkephalin
Dynorphin A
G protein, GTP binding protein
k-Type
Opioid receptor
PCR, polymerase chain reaction
RT, reverse transcription
U50488H
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Summary:We cloned a cDNA for the rat K-opioid receptor from a rat thalamus cDNA library. The deduced amino acid sequence consists of 380 residues with features shared by members of the G protein-coupled receptor family. The specific binding of [ 3H]bremazocine to the membrane of COS-7 cells transfected with the cDNA was displaced by k-specific opioid ligands, but not by μ- and δ-specific ligands. Xenopus oocytes injected with the in vitro transcribed mRNA responded to opioid ligands with the same subtype specificity. Northern blot analysis demonstrated that k-opioid receptor mRNA is expressed in a regionally specific manner in rat brain.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)80240-U