Reaction of nitric oxide with hydrogen peroxide to produce potentially cytotoxic singlet oxygen as a model for nitric oxide-mediated killing

Nitric oxide, as well as being a major regulator of vascular reactivity, has been shown to be one of the mediators of cytotoxicity in macrophages. This cytotoxic effect seems to be due to the interaction between nitric oxide and oxygen-related free radicals. This study shows that, in vitro, nitric o...

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Bibliographic Details
Published in:FEBS letters 1993-04, Vol.321 (1), p.59-62
Main Authors: Noronha-Dutra, Alberto A., Epperlein, Monica M., Woolf, Neville
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell metabolism, cell oxidation
Cell physiology
Cytotoxicity
Cytotoxins
Fundamental and applied biological sciences. Psychology
Hydrogen peroxide
Hydrogen Peroxide - chemistry
In Vitro Techniques
Luminescent Measurements
Molecular and cellular biology
Nitric oxide
Nitric Oxide - chemistry
Oxygen - chemistry
Reactive Oxygen Species - chemistry
Singlet oxygen
Superoxide
Superoxides - chemistry
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Summary:Nitric oxide, as well as being a major regulator of vascular reactivity, has been shown to be one of the mediators of cytotoxicity in macrophages. This cytotoxic effect seems to be due to the interaction between nitric oxide and oxygen-related free radicals. This study shows that, in vitro, nitric oxide reacts with hydrogen peroxide to release large amounts of chemiluminescence with the characteristics of the highly cytotoxic species, singlet oxygen. This is supported by the observation that when nitric oxide was added to a Superoxide generating system, catalase inhibited the production of singlet oxygen while Superoxide dismutase enhanced it.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)80621-Z