Metabolic pathways in Ehrlich ascites tumor cells recovering from a low bioenergetic status

• Published in: FEBS letters 1994-08, Vol.350 (2), p.183-186
• Main Author: Dell'Antone, Paolo
• Format: Article
• Language: English
• Subjects: Acridine orange; Acridine Orange - metabolism; Adenosine Triphosphate - metabolism; Animals; AO, acridine orange; ATP-ase, adenosine triphosphatase; Biological Transport; Carcinoma, Ehrlich Tumor - metabolism; Crabtree effect; EATC, Ehrlich ascites tumor cells; Ehrlich ascites tumor cell; Energy Metabolism; Glucose - metabolism; HEPES, N-2 hydroxyethylpiperazine-N′-2-ethanesulfonic acid; Hydrogen-Ion Concentration; In Vitro Techniques; Male; Membrane Potentials; Mice; Mice, Inbred BALB C; Time Factors; Tumor Cells, Cultured; Tumor metabolism
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(94)00759-4

Ehrlich ascites tumor cells were found to be in a low bioenergetic status, as evaluated by acridine orange uptake and ATP content, when resuspended in a glucose medium shortly after removal from the animal. Dye uptake as well as ATP content then increased for about 2 h at room temperature. This effect was only slightly inhibited by oligomycin. Cells resuspended in a glucose-free medium initially showed high dye uptake and ATP level, which were stable over time: in this case oligomycin caused a drop in both dye uptake and ATP level. The above findings, which are indicative of a marked Crabtree effect in Ehrlich ascites tumor cells, means that it is unlikely that limiting ADP and P i play an important role in the glucose-induced inhibition of oxidative phosphorylation in this system.