A herbicide antidote (safener) induces the activity of both the herbicide detoxifying enzyme and of a vacuolar transporter for the detoxified herbicide

In plants potentially toxic compounds are ultimately deposited in the large central vacuole. In this report we show that isolated barley mesophyll vacuoles take up the glucoside conjugate of the herbicide derivate [5-hydroxyphenyl]primisulfuron. Transport is stimulated by Mg-ATP and is distinct from...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1994-09, Vol.352 (2), p.219-221
Main Authors: Gaillard, Cécile, Dufaud, Annie, Tommasini, Roberto, Kreuz, Klaus, Amrhein, Nikolaus, Martinoia, Enrico
Format: Article
Language:English
Subjects:
Acetamides - metabolism
Biological Transport - drug effects
Biological Transport - physiology
Detoxification
Esculin - pharmacology
Glucose - analogs & derivatives
Glucose - metabolism
Glucoside
Glutathione
Glutathione - analogs & derivatives
Glutathione - metabolism
Glutathione Transferase - metabolism
GST, glutathione-S-transferase
Herbicide
Herbicides - metabolism
Herbicides - pharmacology
Hordeum - metabolism
HPS-G, hydroxy-primisulfuron-glucoside
Inactivation, Metabolic
Kinetics
MOC-GS, metolachlor-glutathione
Naphthalenes - pharmacology
Quinolines
Safener
Salicylates - pharmacology
Salicylic Acid
Transport
Vacuole
Vacuoles - metabolism
Xenobiotics - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In plants potentially toxic compounds are ultimately deposited in the large central vacuole. In this report we show that isolated barley mesophyll vacuoles take up the glucoside conjugate of the herbicide derivate [5-hydroxyphenyl]primisulfuron. Transport is stimulated by Mg-ATP and is distinct from that previously described for glutathione conjugates. Treatment of barley with different herbicide antidotes (safeners) revealed that the safener cloquintocet-mexyl doubles the vacuolar transport activities for both the glutathione and glucoside conjugates. Stimulation of the uptake of the metolachlor—glutathione conjugate was the result of an increased uptake velocity whereas the K m remained unaltered, suggesting that the higher activity was due to a higher expression of the transporter. These results indicate that modulation of vacuolar transport activities are an integral part of the detoxification mechanism of plants.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)00961-9