Elevated cytosolic free Ca 2+ concentrations and massive Ca 2+ accumulation within vacuoles, in yeast mutant lacking PMR1, a homolog of Ca 2+-ATPase

The Ca 2+-ATPase homolog of Saccharomyces cerevisiae, PMR1, cloned by Rudolph et al. (Cell 58 (1989) 133–145) is required for normal Golgi functions. We have investigated the role of Pmr1-protein in maintaining homeostasis of cytosolic free Ca 2+ concentration ([Ca 2+] i). It was found that exposure...

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Bibliographic Details
Published in:FEBS letters 1996-08, Vol.392 (2), p.194-200
Main Authors: Halachmi, D., Eilam, Y.
Format: Article
Language:English
Subjects:
Ca 2+-ATPase
Cytosolic Ca 2+ homeostasis
Golgi
PMR1 gene
Saccharomyces cerevisiae
Vacuolar Ca 2
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Summary:The Ca 2+-ATPase homolog of Saccharomyces cerevisiae, PMR1, cloned by Rudolph et al. (Cell 58 (1989) 133–145) is required for normal Golgi functions. We have investigated the role of Pmr1-protein in maintaining homeostasis of cytosolic free Ca 2+ concentration ([Ca 2+] i). It was found that exposure to moderately high Ca 2+ concentrations led to elevated levels of [Ca 2+] i in cells of pmr1 null mutant, in comparison with cells of pmr2 isogenic mutant (defective in cell-membrane Na +-ATPase) and of an isogenic wild type. In addition, we showed that PMR1 deletion causes massive accumulation of Ca 2+ in the vacuoles and affects the rates of Ca 2+ influx and efflux.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)00799-5