Determination of 3-phenyl-2-thiohydantoin-amino acids by means of in situ quantitation of thin-layer chromatograms

This investigation demonstrates the applicability of the linear detector system for the quantitation or 3-phenyl-2-thiohydantoin (PTH)-amino acids on thin-layer chromatograms. This method is rapid and more sensitive than the Edman procedure. As little as 0.5 nanomoles of PTH-amino acid can be determ...

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Bibliographic Details
Published in:Journal of Chromatography A 1975-04, Vol.107 (2), p.311-315
Main Authors: Zwolinski, G.K., Treiber, L.R.
Format: Article
Language:English
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Summary:This investigation demonstrates the applicability of the linear detector system for the quantitation or 3-phenyl-2-thiohydantoin (PTH)-amino acids on thin-layer chromatograms. This method is rapid and more sensitive than the Edman procedure. As little as 0.5 nanomoles of PTH-amino acid can be determined quantitatively. Standard calibrations are presented for a representative group of PTH-amino acids with correlation coefficients r > 0.996 and standard deviation s a < 3.14%.
ISSN:0021-9673
DOI:10.1016/0021-9673(75)80006-9