Resolution of enzymes that hydrolyse phosphate esters from agave cantala on amberlite IR-120 (Al 3+)

A technique for the resolution of functionally similar enzymes is described. Enzymes that hydrolyse phosphate esters, viz., 3′-nucleotidase, pyrophosphatase, phosphatase, and ribonuclease, from Agave cantala have been resolved on an IR-120 (Al 3+) column free from each other, and their eventual puri...

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Bibliographic Details
Published in:Journal of Chromatography A 1979, Vol.168 (2), p.495-499
Main Authors: Sane, A.S., Hegde, M.V., Joshi, P.N.
Format: Article
Language:English
Subjects:
plant biochemistry
plant physiology
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Summary:A technique for the resolution of functionally similar enzymes is described. Enzymes that hydrolyse phosphate esters, viz., 3′-nucleotidase, pyrophosphatase, phosphatase, and ribonuclease, from Agave cantala have been resolved on an IR-120 (Al 3+) column free from each other, and their eventual purification thereby obtained is described.
ISSN:0021-9673
DOI:10.1016/0021-9673(79)80021-7