High-performance liquid chromatographic determination of valproic acid in plasma using a micelle-mediated pre-column derivatization

A method for the determination of valproic acid (2-propylpentanoic acid) in plasma by high-performance liquid chromatography (HPLC) after pre-column derivatization is described. The derivatization of valproic acid with a fluorophore and UV label, 4-bromomethyl-7-methoxycoumarin, is performed in plas...

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Bibliographic Details
Published in:Journal of Chromatography A 1988-12, Vol.456, p.191-199
Main Authors: van der Horst, F.A.L., Eikelboom, G.G., Holthuis, J.J.M.
Format: Article
Language:English
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Summary:A method for the determination of valproic acid (2-propylpentanoic acid) in plasma by high-performance liquid chromatography (HPLC) after pre-column derivatization is described. The derivatization of valproic acid with a fluorophore and UV label, 4-bromomethyl-7-methoxycoumarin, is performed in plasma diluted with an aqueous micellar system. No extraction or solvent evaporation steps are required. The mechanism of the derivatization of the carboxylic acid is based on phase-transfer catalysis. The sample preparation, including the derivatization step, is rapid and very simple. the proposed HPLC-method was evaluated and compared with a standard immunological assay used for the determination of valproic acid in plasma.
ISSN:0021-9673
DOI:10.1016/0021-9673(86)80018-8