Fast and sensitive staining technique for catalase in polyacrylamide gel

The described staining technique for catalase activity on polyacrylamide gel leads to sharp colourless bands on a uniform brown backgroud. It is based on the oxidation of a-dianisidine with hydrogen peroxide by the catalytic action of haemin. Catalase activity can be detected down to 0.25 U. The who...

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Bibliographic Details
Published in:Journal of Chromatography A 1992-03, Vol.594 (1), p.400-402
Main Authors: Pichorner, Heidi, Loew, Sonja, Korori, Soudabeh A.A., Jessner, Georg, Ebermann, Robert
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
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Summary:The described staining technique for catalase activity on polyacrylamide gel leads to sharp colourless bands on a uniform brown backgroud. It is based on the oxidation of a-dianisidine with hydrogen peroxide by the catalytic action of haemin. Catalase activity can be detected down to 0.25 U. The whole staining procedure needs about 30 min and parallel detection of peroxidase activity is possible.
ISSN:0021-9673
DOI:10.1016/0021-9673(92)80356-Y