High-performance liquid chromatographic determination of 2-furaldehyde in spirits

• Published in: Journal of Chromatography A 1992-01, Vol.590 (2), p.235-240
• Main Authors: Lo Coco, Filippo, Ceccon, Luciano, Valentini, Clemente, Novelli, Veronica
• Format: Article
• Language: English
• Subjects: 2,4-dinitrophenylhydrazones; alcoholic beverages; Biological and medical sciences; carbonyl compounds; determination; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; high performance liquid chromatography; Methods of analysis, processing and quality control, regulation, standards
• ISSN: 0021-9673
• DOI: 10.1016/0021-9673(92)85386-8

Official methods for the determination of 2-furaldehyde in spirits involve for a spectrophotometric evaluation, which is characterized by poor specificity. Gas chromatographic evaluations have also been proposed, which offer a much higher sensitivity, particularly when capillary columns are used. In this paper a high-performance liquid chromatographic (HPLC) method based on the formation of the 2,4-dinitrophenylhydrazones of carbonyl compounds and subsequent reversed-phase separation of these derivatives is described. Derivatization is carried out by utilizing an acidic solution of 2,4-dinitrophenylhydrazine in acetonitrile. Precipitation of the derivatives is avoided, and direct injection of the sample into the HPLC system is allowed. The determination ofrers a high specificity and a detection limit of the order of 10 −8 mol/1. Accuracy and reproducibility data are presented.