Monitoring excitatory amino acid release in vivo by microdialysis with capillary electrophoresis- electrochemistry

Capillary electrophoresis (CE) with electrochemical detection (ED) was used to determine extracellular levels of aspartate, glutamate and alanine in samples from the frontoparietal cortex of the rat which were obtained by microdialysis. The method was used to monitor the effect on the overflow of th...

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Bibliographic Details
Published in:Journal of Chromatography A 1992-09, Vol.608 (1), p.189-195
Main Authors: O'Shea, Thomas J., Weber, Paul L., Bammel, Brad P., Lunte, Craig E., Lunte, Susan M., Smyth, Malcolm R.
Format: Article
Language:English
Subjects:
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Proteins
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Summary:Capillary electrophoresis (CE) with electrochemical detection (ED) was used to determine extracellular levels of aspartate, glutamate and alanine in samples from the frontoparietal cortex of the rat which were obtained by microdialysis. The method was used to monitor the effect on the overflow of the excitatory amino acids aspartate and glutamate of an influx of high concentrations of potassium ion. Samples were derivatized with naphthalenedialdehyde-cyanide prior to analysis. Detection limits for aspartate and glutamate were 80 and 100 n M, respectively. CEî—¸ED is extremely useful for the analysis of microdialysis samples because of the very small sample volumes required by this analytical technique. The use of ED provides the requisite sensitivity and allows verification of peak purity by voltammetry.
ISSN:0021-9673
DOI:10.1016/0021-9673(92)87123-P