Determination of fatty acids in fish oil dietary supplements by capillary liquid chromatography with laser-induced fluorescence detection

The 4-bromomethyl-7-methoxycoumarin derivatives of 14 saturated fatty acids, including the ω - 3 fatty acids, were separated by reversed-phase liquid chromatography and detected by laser-induced fluorescence. Baseline resolution was obtained by using a high-efficiency packed capillary column with 24...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1992-12, Vol.627 (1), p.87-96
Main Authors: Shin Yoo, Jong, MacGuffin, Victoria L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
determination
fatty acids
fish oils
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
liquid chromatography
Methods of analysis, processing and quality control, regulation, standards
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The 4-bromomethyl-7-methoxycoumarin derivatives of 14 saturated fatty acids, including the ω - 3 fatty acids, were separated by reversed-phase liquid chromatography and detected by laser-induced fluorescence. Baseline resolution was obtained by using a high-efficiency packed capillary column with 240 000 theoretical plates, together with a systematic optimization of the mobile phase composition. The retention indices of the fatty acid derivatives correlated well with a predictive empirical model, showing accuracy better than 0.46% relative error and reproducibility better than ±0.1% relative standard deviation. The physiologically important fatty acids with 12–22 carbon atoms and 0–6 double bonds were determined at the femtomole level in fish oil dietary supplements by using this methodology.
ISSN:0021-9673
DOI:10.1016/0021-9673(92)87189-F