High-performance liquid chromatographic determination of the geometrical isomers of β-carotene in several foodstuffs

To better evaluate the provitamin A content of foodstuffs, an HPLC separation of the geometric isomers of β-carotene was developed. Of the several different stationary phases studied, the best separations of all-trans-9- cis- and 13- cis-β-carotene were obtained with calcium hydroxide columns using...

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Bibliographic Details
Published in:Journal of Chromatography A 1995-04, Vol.697 (1), p.289-294
Main Authors: Carvalho, Cassia R.L., Carvalho, Paulo Roberto N., Collins, Carol H.
Format: Article
Language:English
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Summary:To better evaluate the provitamin A content of foodstuffs, an HPLC separation of the geometric isomers of β-carotene was developed. Of the several different stationary phases studied, the best separations of all-trans-9- cis- and 13- cis-β-carotene were obtained with calcium hydroxide columns using isocratic elution with n-hexane, isooctane or mixtures of these solvents. When xanthophyll pigments are also present, a gradient elution with acetone in isooctane is required. These techniques were used to determine the provitamin A content of squash, peaches, dendeˆoil (palm tree oil) and orange juice.
ISSN:0021-9673
DOI:10.1016/0021-9673(94)00752-U