Quantitative estimation of heterocyclic aromatic amines by ion-exchange chromatography and electrochemical detection

Conditions for the quantitative determination of three heterocyclic aromatic amines (HAAs), produced by heat processing of protein-rich food products, were established for a HPLC-electrochemical detection system. The separation of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimi...

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Bibliographic Details
Published in:Journal of Chromatography A 1995-04, Vol.697 (1), p.377-382
Main Authors: Van Dyck, M.M.C., Rollmann, B., De Meester, C.
Format: Article
Language:English
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Summary:Conditions for the quantitative determination of three heterocyclic aromatic amines (HAAs), produced by heat processing of protein-rich food products, were established for a HPLC-electrochemical detection system. The separation of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQ x) was achieved on an ion-exchange column with acetonitrile-80 m M phosphate buffer (30:70) mobile phase at pH 5.6. The figures of merit were calculated. Reproducibility gave a relative standard deviation of 1.6–2.4% when measured by peak area. Detection limits (signal-to-noise ratio 3) ranged from 35 pg for MeIQ x to 70 pg for MeIQ. The method was applied to the determination of HAAs in a commercial beef extract sample.
ISSN:0021-9673
DOI:10.1016/0021-9673(94)00837-Y