Simultaneous sorption and analytical derivatization on a polystyrene-divinylbenzene polymer preparation of chromophoric and fluorophoric derivatives of the prostaglandins

A solid phase consisting of a macroreticular styrene-divinylbenzene polymer was used as a support for the sorption and derivatization of the prostaglandins to form chromophoric and fluorophoric esters. These highly potent physiological mediators are a group of 20 carbon, oxygenated carboxylic acids...

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Bibliographic Details
Published in:Journal of Chromatography A 1995-02, Vol.691 (1), p.231-237
Main Authors: Rosenfeld, Jack M., Fang, Xingchun
Format: Article
Language:English
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Summary:A solid phase consisting of a macroreticular styrene-divinylbenzene polymer was used as a support for the sorption and derivatization of the prostaglandins to form chromophoric and fluorophoric esters. These highly potent physiological mediators are a group of 20 carbon, oxygenated carboxylic acids which at neutral pH are highly water soluble. In addition to functioning as a support for the simultaneous isolation and derivatization, the solid phase also retained the derivatized analytes while a substantial amount of excess reagent was selectively eluted. The final clean-up was by semi-preparative chromatography.
ISSN:0021-9673
DOI:10.1016/0021-9673(94)01025-A