Efficiency of chemical oligonucleotide synthesis evaluated by ion-exchange high-performance liquid chromatography

Ion-exchange high-performance liquid chromatography (HPIEC) is a method of choice for the separation of crude solid-phase synthesized oligonucleotides. It shows peaks for target and error sequences and it is used for quantification of oligonucleotide synthesis. This paper presents a homeodynamic mod...

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Bibliographic Details
Published in:Journal of Chromatography A 1995-07, Vol.706 (1), p.405-419
Main Authors: Földes-Papp, Zeno, Birch-Hirschfeld, Eckhard, Rösch, Rudi, Hartmann, Manfred, Kleinschmidt, Albrecht K., Seliger, Hartmut
Format: Article
Language:English
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Summary:Ion-exchange high-performance liquid chromatography (HPIEC) is a method of choice for the separation of crude solid-phase synthesized oligonucleotides. It shows peaks for target and error sequences and it is used for quantification of oligonucleotide synthesis. This paper presents a homeodynamic model of polymer-supported oligonucleotide synthesis and a numerical solution, which accounts for elution profiles obtained by HPIEC. The elution profiles are consistent with the model. The chain-length distributions for 30mer syntheses on different support materials were also studied and the probability moment analysis was applied as a quantitative measure of the differential relationship of fractal dimension. The support systems for chemical synthesis of short oligonucleotides are classified according to this relationship.
ISSN:0021-9673
DOI:10.1016/0021-9673(94)01221-Y