Characterization of charge-modified and fluorescein-labeled antibody by capillary electrophoresis using laser-induced fluorescence Application to immunoassay of low level immunoglobulin A

An affinity-purified and fluorescein-labeled antibody was chemically modified to alter its electrophoretic mobility. The functional activity of the resulting modified and fluor labeled antibody appeared was preserved. Immunoassay is carried out by the addition of an appropriate amount of the labeled...

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Bibliographic Details
Published in:Journal of Chromatography A 1994-10, Vol.680 (2), p.419-423
Main Author: Chen, Fu-Tai A.
Format: Article
Language:English
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Summary:An affinity-purified and fluorescein-labeled antibody was chemically modified to alter its electrophoretic mobility. The functional activity of the resulting modified and fluor labeled antibody appeared was preserved. Immunoassay is carried out by the addition of an appropriate amount of the labeled antibody to a sample containing the antigen. Separation of the antigen-bound and free labeled antibody was performed by capillary electrophoresis with laser- induced fluorescence detection. Both free and antigen-bound labeled antibody species may be analysed simultaneously. Quantitation of the immunoassay is achieved with an on-line data analysis.
ISSN:0021-9673
DOI:10.1016/0021-9673(94)85138-7