Light scattering in mixtures of BSA, BSA dimers, and fibrinogen under the influence of electric fields

We report the development of a new technique for analyzing a mixture of different charged macromolecules. This technique combines light scattering and electrophoresis to permit the simultaneous determination of the electrophoretic mobilities and diffusion coefficients of charged macromolecules in so...

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Bibliographic Details
Published in:Journal of colloid and interface science 1972-01, Vol.39 (3), p.670-675
Main Authors: Ware, B.R, Flygare, W.H
Format: Article
Language:English
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Summary:We report the development of a new technique for analyzing a mixture of different charged macromolecules. This technique combines light scattering and electrophoresis to permit the simultaneous determination of the electrophoretic mobilities and diffusion coefficients of charged macromolecules in solution. High resolution experiments on bovine serum albumin (BSA) have provided both the electrophoretic mobility and diffusion coefficient within a standard deviation of 5%. We report also the first observation of analytical separation of multicomponent systems by this technique. Distinct resolution for the systems BSA-BSA dimers and BSA-BSA dimersfibrinogen has been achieved. Finally we discuss potential extensions and applications of this technique.
ISSN:0021-9797
1095-7103
DOI:10.1016/0021-9797(72)90075-6