Crystallization and preliminary X-ray analysis of nerve growth factor from Vipera lebetina

Crystals of nerve growth factor purified from Vipera lebetina venom have been grown by the vapour diffusion method from concentrated solutions of ammonium sulphate. The crystals, which have the form of trigonal, elongated prisms (0.3×0.3×1.0 mm), diffract X-rays to at least 3.2 Å resolution and belo...

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Bibliographic Details
Published in:Journal of crystal growth 1991-03, Vol.110 (1), p.214-219
Main Authors: Joachimiak, A., Giel, M., Otwinowski, Z., Arumae, U., Siigur, J., Saarma, M., Sigler, P.B., Barciszewski, J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Crystalline structure
Fundamental and applied biological sciences. Psychology
Molecular biophysics
Structure in molecular biology
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Summary:Crystals of nerve growth factor purified from Vipera lebetina venom have been grown by the vapour diffusion method from concentrated solutions of ammonium sulphate. The crystals, which have the form of trigonal, elongated prisms (0.3×0.3×1.0 mm), diffract X-rays to at least 3.2 Å resolution and belong to the R32 space group (rhombohedral packing). The hexagonal unit cell parameters have been determined from precession and oscillation photographs as follows: a = b = 301.1 A ̊ , c = 247.5 A ̊ , α = β =90°, γ=120°, V = 1.08×10 6 A ̊ 3 . The crystalline protein retains its activity when tested in vitro.
ISSN:0022-0248
1873-5002
DOI:10.1016/0022-0248(91)90888-C