Xanthine and hypoxanthine sensors based on xanthine oxidase immobilized in poly(mercapto-p-benzoquinone) film

The construction and performance of an enzyme electrode as an amperometric sensor of xanthine and hypoxanthine is described. Xanthine oxidase has been immobilized in a conductive redox polymer, poly(mercapto-p-benzoquinone), by means of an electropolymerization of mercaptohydroquinone in the presenc...

Full description

Saved in:
Bibliographic Details
Published in:Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 1996-07, Vol.410 (2), p.173-179
Main Authors: Arai, Gorou, Takahashi, Shigeo, Yasumori, Iwao
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Chemistry
Electrochemistry
Electrodes: preparations and properties
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General and physical chemistry
Hypoxanthine
Ion selective electrodes
Methods. Procedures. Technologies
Polymer film
Various methods and equipments
Xanthine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The construction and performance of an enzyme electrode as an amperometric sensor of xanthine and hypoxanthine is described. Xanthine oxidase has been immobilized in a conductive redox polymer, poly(mercapto-p-benzoquinone), by means of an electropolymerization of mercaptohydroquinone in the presence of the enzyme. An Au-electroplated glassy carbon electrode coated with the resulting polymer film functioned well as a direct response type of sensor, where the polymer chain served as a conductive molecular chain between the active sites in the enzyme and the substrate electrode. Response characteristics as well as kinetic parameters have been evaluated.
ISSN:1572-6657
1873-2569
DOI:10.1016/0022-0728(96)04553-6