Fluorescence lifetime measurements under a microscope by the time-correlated single-photon counting technique

Instrumentation for the fluorescence lifetime measurements under a microscope and with sub-nanosecond time resolution is described. The present technique was applied to clover leaves stained with acridine orange, and to three-layered polyvinyl alcohol (PVA) films which is consisted of erythrosine B...

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Bibliographic Details
Published in:Journal of luminescence 1986-08, Vol.35 (5), p.247-253
Main Authors: Minami, Takaaki, Kawahigashi, Masaki, Sakai, Yasuhide, Shimamoto, Katsuyuki, Hirayama, Satoshi
Format: Article
Language:English
Subjects:
Applied sciences
Exact sciences and technology
Physicochemistry of polymers
Polymers and radiations
Properties and characterization
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Summary:Instrumentation for the fluorescence lifetime measurements under a microscope and with sub-nanosecond time resolution is described. The present technique was applied to clover leaves stained with acridine orange, and to three-layered polyvinyl alcohol (PVA) films which is consisted of erythrosine B (4.7 × 10 -3 M) and rhodamine B (3.1 × 10 -3 M) layers (3 μ m each in thickness) separated by a blank PVA film of 3–6 μ m in thickness. Detailed analysis of the fluorescence decay curves has shown that it is possible to distinguish a small section spatially ( ⋍ 3 μ m in a vertical as well as horizontal direction) in terms of the fluorescence lifetime.
ISSN:0022-2313
1872-7883
DOI:10.1016/0022-2313(86)90047-5