Three-dimensional homonuclear TOCSY-NOESY of nucleic acids. Possibilities for improved assignments

The application of 3D TOCSY-NOESY spectroscopy to the analysis of nucleic acids is presented, using a TOCSY-NOESY spectrum of the DNA hairpin 5′GTTCCA-AAC-TGGAAC3′. An easy and concise method for describing the magnetization transfer in 3D experiments as applied to the TOCSY-NOESY experiment of nucl...

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Bibliographic Details
Published in:Journal of magnetic resonance (1969) 1991, Vol.94 (1), p.101-111
Main Authors: Mooren, M.M.W, Hilbers, C.W, Van Der Marel, G.A, Van Boom, J.H, Wijmenga, S.S
Format: Article
Language:English
Subjects:
Atomic and molecular physics
Exact sciences and technology
Molecular properties and interactions with photons
Physics
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Summary:The application of 3D TOCSY-NOESY spectroscopy to the analysis of nucleic acids is presented, using a TOCSY-NOESY spectrum of the DNA hairpin 5′GTTCCA-AAC-TGGAAC3′. An easy and concise method for describing the magnetization transfer in 3D experiments as applied to the TOCSY-NOESY experiment of nucleic acids is presented. It is shown that a 3D TOCSY-NOESY experiment can be considered as a collection of spin-lock patterns. In particular the repeating spin-lock pattern present in the f 1 f 3 planes is an extremely useful feature of a TOCSY-NOESY experiment since it can be used to distinguish between protons whose resonances overlap. The application of TOCSY-NOESY makes it possible also to assign the very crowded H4′, H5′, H5″ resonances.
ISSN:0022-2364
1557-8968
DOI:10.1016/0022-2364(91)90297-7