Intracellular localization of geranylpyrophosphate synthase from cell cultures of Lithospermum erythrorhizon

We have investigated the subcellular localization of the geranylpyrophosphate (GPP) synthase (EC 2.5.1.1) in Lithospermum erythrorhizon cell cultures using a cell fractionation procedure followed by comparison to marker enzyme activities and immunodetection in Western blots. The results demonstrate...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 1995-02, Vol.38 (3), p.623-627
Main Authors: Sommer, Susanne, Severin, Klaus, Camara, Bilal, Heide, Lutz
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell biochemistry
Cell physiology
Fundamental and applied biological sciences. Psychology
General pharmacology
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant physiology and development
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Summary:We have investigated the subcellular localization of the geranylpyrophosphate (GPP) synthase (EC 2.5.1.1) in Lithospermum erythrorhizon cell cultures using a cell fractionation procedure followed by comparison to marker enzyme activities and immunodetection in Western blots. The results demonstrate the localization of the enzyme within the cytosol. This contrasts to the plastidial localization of the GPP synthase in Vitis vinifera. This discrepancy is discussed in regard to the different metabolic pathways in which GPP is involved in these two plant species.
ISSN:0031-9422
1873-3700
DOI:10.1016/0031-9422(94)00684-L