5-epi-Aristolochene 3-hydroxylase from green pepper

5-epi-Aristolochene 3-hydroxylase, one of the key enzymes of the biosynthetic pathway leading to capsidiol, a major phytoalexin of green pepper ( Capsicum annuum), is localized in the microsomes of elicitor treated green pepper fruit. The enzyme was found to be a P-450-dependent enzyme with an estim...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 1995-02, Vol.38 (3), p.609-613
Main Authors: Hoshino, Tamotsu, Yamaura, Takao, Imaishi, Hiromasa, Chida, Manami, Yoshizawa, Yuko, Higashi, Ken, Ohkawa, Hideo, Mizutani, Junya
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Pathology, epidemiology, host-fungus relationships. Damages, economic importance
Phytopathology. Animal pests. Plant and forest protection
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Summary:5-epi-Aristolochene 3-hydroxylase, one of the key enzymes of the biosynthetic pathway leading to capsidiol, a major phytoalexin of green pepper ( Capsicum annuum), is localized in the microsomes of elicitor treated green pepper fruit. The enzyme was found to be a P-450-dependent enzyme with an estimated molecular mass of 59 kDa. The total microsomal P-450 content increased and the λ max of the CO-binding spectrum changed in response to elicitation. Immunoblot analysis of fruit microsomal proteins was performed with anti-tulip constitutive P-450 antibody during the elicitation period. A 48 kDa protein strongly immunoreacted with the P-450 antibody, and a decrease of this P-450-like 48 kDa protein was observed with time. These results suggest that elicitation induces the production of P-450 isoforms.
ISSN:0031-9422
1873-3700
DOI:10.1016/0031-9422(94)00695-P