Penicillin acylase extraction by osmotic shock

Penicillin acylase was extracted from E. Coli by osmotic shock. The process was optimized by factorial design, scaled up and integrated to a purification process in order to compare it with purification processes reported in the literature. A specific parameter PPEF (Purification Processes Evaluatio...

Full description

Saved in:
Bibliographic Details
Published in:Process biochemistry (1991) 1992-07, Vol.27 (4), p.217-223
Main Authors: Rodriguez, M., Güereca, L., Valle, F., Quintero, R., López-Munguia, A.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Penicillin acylase was extracted from E. Coli by osmotic shock. The process was optimized by factorial design, scaled up and integrated to a purification process in order to compare it with purification processes reported in the literature. A specific parameter PPEF (Purification Processes Evaluation Factor) was defined for this purpose.
ISSN:1359-5113
1873-3298
DOI:10.1016/0032-9592(92)80021-T