Improvement of the thermostability of β-Galactosidase from Kluyveromyces marxianus

Comparative studies on some preparations of Kluyveromyces marxianus β-galactosidase show that enzyme localized in permeabilized cells and in glutaraldehyde treated permeabilized cells was more thermostable at 45°C, than cell-free enzyme. In contrast, the temperature optimum (50-52°C) was not affecte...

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Bibliographic Details
Published in:Process biochemistry (1991) 1995, Vol.30 (7), p.649-652
Main Authors: Tomáška, M., Stredǎnský, M., Gemeiner, P., Šturdík, E.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Miscellaneous
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Summary:Comparative studies on some preparations of Kluyveromyces marxianus β-galactosidase show that enzyme localized in permeabilized cells and in glutaraldehyde treated permeabilized cells was more thermostable at 45°C, than cell-free enzyme. In contrast, the temperature optimum (50-52°C) was not affected significantly by the intracellular localization. Permeabilized cells immobilized by entrapment into hardened calcium alginate gel or treated with glutaraldehyde were used for repeated lactose hydrolysis at 45°C and showed good operational stability.
ISSN:1359-5113
1873-3298
DOI:10.1016/0032-9592(94)00056-5