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Immobilization of Bacillus stearothermophilus Cells by Entrapment in Various Matrices

Bacillus stearothermophilus G-82 cells were immobilized by entrapment in alginate, agarose, agar, carrageenan, polyurethane and polyacrylamide. Polyacrylamide (20%) was the best support for immobilization, providing relatively high pullulanase production with minimal appearance of free cells in the...

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Bibliographic Details
Published in:Process biochemistry (1991) 1995, Vol.30 (2), p.141-144
Main Authors: Manolov, R.J., Kambourova, M.S., Emanuilova, E.I.
Format: Article
Language:English
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Summary:Bacillus stearothermophilus G-82 cells were immobilized by entrapment in alginate, agarose, agar, carrageenan, polyurethane and polyacrylamide. Polyacrylamide (20%) was the best support for immobilization, providing relatively high pullulanase production with minimal appearance of free cells in the medium. Fifteen-fold elevation of specific enzyme activity in culture liquid was observed for the immobilized system.
ISSN:1359-5113
1873-3298
DOI:10.1016/0032-9592(95)80004-2