A gas perfusion microcalorimeter for studies of plant tissue

A gas perfusion microcalorimetric system has been developed for metabolic investigations of plant tissues under dark conditions. The system consists of two twin heat conduction microcalorimeters connected by gas-tight tubing. During measurements, a flow of humid gas enters the main calorimeter conta...

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Bibliographic Details
Published in:Thermochimica acta 1995-03, Vol.251, p.323-333
Main Authors: Bäckman, P., Breidenbach, R.W., Johansson, P., Wadsö, I.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gas perfusion microcalorimeter
General aspects, methods
Microcalorimetry
Plant physiology and development
Potato
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Summary:A gas perfusion microcalorimetric system has been developed for metabolic investigations of plant tissues under dark conditions. The system consists of two twin heat conduction microcalorimeters connected by gas-tight tubing. During measurements, a flow of humid gas enters the main calorimeter containing the plant tissue. The volume of this sample chamber can be varied between 4 and 25 ml and the flow rate of the gas can be varied up to maximum of 150 ml h −1. The outgoing air is allowed to pass through a second calorimeter, serving as an on-line CO 2 analyzer. Gases, such as NO x or CO 2, can be added to the incoming gas-flow either continuously or by stepwise injection. The calorimetric system has been tested in respiratory experiments with potato tuber slices.
ISSN:0040-6031
1872-762X
DOI:10.1016/0040-6031(94)02144-D