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Light-induced electrical responses of dried chromatophore film: effect of the addition of cytochrome c

Chromatophores, photosynthetic membranes of photosynthetic bacteria, were isolated and cast on a transparent electrode to form films. The absorption spectrum of the film was similar to that of the native chromatophore. This indicated that the chromophore-protein structures were maintained through dr...

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Bibliographic Details
Published in:Thin solid films 1989-11, Vol.180 (1), p.85-88
Main Authors: Majima, Toshikazu, Miyake, Jun, Hara, Masayuki, Ajiki, Shu-Ichi, Sugino, Hiroaki, Toyotama, Hideki
Format: Article
Language:English
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Summary:Chromatophores, photosynthetic membranes of photosynthetic bacteria, were isolated and cast on a transparent electrode to form films. The absorption spectrum of the film was similar to that of the native chromatophore. This indicated that the chromophore-protein structures were maintained through drying. Electrical transients of the film on the light pulsed excitation were examined. The amplitude and decay of the electrical transient of the film were altered by the addition of horse heart cytochrome c to the chromatophore of Rhodobacter sphaeroides R-26, which lacks the reaction centre-bound cytochrome c. The addition of cytochrome c into the film resulted in an increase in the light-induced current and a decrease in current decay.
ISSN:0040-6090
1879-2731
DOI:10.1016/0040-6090(89)90057-6