A device for visual detection of antigens and antibodies by means of light interference

A device for visual detection of antigens and antibodies was investigated. The device is composed of a light-reflecting substrate, a light-interfering layer and an immobilized antibody (antigen) layer. The detection is based on the colour change due to light interference before and after reaction of...

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Bibliographic Details
Published in:Thin solid films 1990-10, Vol.191 (2), p.369-381
Main Authors: Kawaguchi, Takeyuki, Shiro, Takashi, Iwata, Kaoru
Format: Article
Language:English
Subjects:
Biological and medical sciences
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
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Summary:A device for visual detection of antigens and antibodies was investigated. The device is composed of a light-reflecting substrate, a light-interfering layer and an immobilized antibody (antigen) layer. The detection is based on the colour change due to light interference before and after reaction of the immobilized antibody (antigen) with antigen (antibody) in an aqueous specimen. Theoretical analysis of the light interference suggested that the application of a light-reflecting layer on the surface of the device after an antigen-antibody reaction is effective for both glancing angle and clarity. Colloidal gold was found to be a good candidate for the layer. With a device of Ferrotype TM/SiO 2(1000 Å)/(mixed monomolecular film of anti-human IgG and methyl arachidate), 1 × 10 −7 g ml −1 of human IgG could be visually detected from the normal direction of the device.
ISSN:0040-6090
1879-2731
DOI:10.1016/0040-6090(90)90387-S