β-Carboline as a fluorescence standard

Fluorescence measurements of β-carboline in 1 N H 2SO 4 suggest its use as a fluorescence reference compound superior to the widely used quinine bisulphate. The emission spectrum (λ max = 450 nm) and quantum yield (φ F = 0.60) of the β-carboline cation are similar to those of quinine. However, in co...

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Bibliographic Details
Published in:Journal of photochemistry 1985-01, Vol.31 (1), p.113-121
Main Authors: Ghiggino, K.P., Skilton, P.F., Thistlethwaite, P.J.
Format: Article
Language:English
Subjects:
Atomic and molecular physics
Exact sciences and technology
Intensities and shapes of molecular spectral lines and bands
Molecular properties and interactions with photons
Physics
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Summary:Fluorescence measurements of β-carboline in 1 N H 2SO 4 suggest its use as a fluorescence reference compound superior to the widely used quinine bisulphate. The emission spectrum (λ max = 450 nm) and quantum yield (φ F = 0.60) of the β-carboline cation are similar to those of quinine. However, in contrast to quinine, the fluorescence decay of β-carboline is an excellent fit to a single-exponential lifetime across the emission band with τ = 22.03 ± 0.12 ns. The experimental conditions required for the application of β-carboline as a fluorescence standard are reported.
ISSN:0047-2670
DOI:10.1016/0047-2670(85)85079-6