A method for the stabilization of toluidine blue vital staining for histological and ultrastructural studies

A method previously developed to stabilize methylene blue vital staining has been applied to toluidine blue, a basic dye with a closely related chemical structure commonly used as a vital stain for the screening of oral and esophageal lesions. The stain was made stable by adding 1% sodium silicotung...

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Bibliographic Details
Published in:Micron 1983, Vol.14 (4), p.353-356
Main Authors: Marnay, J., Herlin, P., Jacob, J.H., Mandard, A.M.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Citations: Items that this one cites
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Summary:A method previously developed to stabilize methylene blue vital staining has been applied to toluidine blue, a basic dye with a closely related chemical structure commonly used as a vital stain for the screening of oral and esophageal lesions. The stain was made stable by adding 1% sodium silicotungstate to an aldehyde fixative (formaldehyde or glutaraldehyde). Sections were prepared for light and electron microscopy.
ISSN:0047-7206
0739-6260
1878-1152
DOI:10.1016/0047-7206(83)90007-9