RNA synthesis in root meristem cells treated with actinomycin D

The effects of actinomycin D (AMD) at different concentrations and periods of treatment on RNA synthesis have been investigated in meristematic cells of Allium cepa L. roots. The analysis was accomplished by electropheresis on SDS agarose gels of nucleic acid extracts from 3H-uridine-labelled cells....

Full description

Saved in:
Bibliographic Details
Published in:Environmental and experimental botany 1985-01, Vol.25 (4), p.293-301
Main Authors: Olmedilla, A., Fernández-Gómez, M.E., Diaz de la Espina, S.Moreno
Format: Article
Language:English
Subjects:
actinomycin D
Allium cepa
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Metabolism
Metabolism. Physicochemical requirements
Plant physiology and development
RNA
root meristems
synthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effects of actinomycin D (AMD) at different concentrations and periods of treatment on RNA synthesis have been investigated in meristematic cells of Allium cepa L. roots. The analysis was accomplished by electropheresis on SDS agarose gels of nucleic acid extracts from 3H-uridine-labelled cells. The rate of uptake of the tritiated antibiotic was also estimated. The results show that AMD impaired ribosomal RNA synthesis in plant cells. As the concentration increased and treatment became longer, the inhibition of synthesis was greater. The delay in the inhibition caused by AMD was not due to a deficient uptake of the antibiotic.
ISSN:0098-8472
1873-7307
DOI:10.1016/0098-8472(85)90027-9