The development of a pollen dispersal system

A pollen dispersal system for rapidly establishing known populations of pollen grains on growth media for in vitro studies is described. The system consists of a mixing chamber where the pollen grains are suspended in an air stream and a dispersal chamber where the pollen grains are directed to the...

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Bibliographic Details
Published in:Environmental and experimental botany 1987, Vol.27 (3), p.325-328
Main Authors: Waldron, Peter F., Craker, Lyle E.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
laboratory techniques
Plant physiology and development
pollen
spread
Tissue cultures, protoplasts
Zea mays
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Summary:A pollen dispersal system for rapidly establishing known populations of pollen grains on growth media for in vitro studies is described. The system consists of a mixing chamber where the pollen grains are suspended in an air stream and a dispersal chamber where the pollen grains are directed to the growth media surface. Pollen from corn plants was used in testing the system. The density and distribution of pollen on the media surface can be adjusted to obtain maximum germination by changing the quantity of pollen applied and the velocity of the air stream, respectively. The system allows the simultaneous inoculation of six Petri plates with pollen.
ISSN:0098-8472
1873-7307
DOI:10.1016/0098-8472(87)90042-6