Ethanol production from cassava starch using a highly productive strain of Zymomonas mobilis and Saccharomyces uvarum ATCC 26602

The liquefaction, simultaneous saccharification and fermentation (SSF) of cassava starch (225 g litre −1 glucose equivalent) were investigated in order to optimize the process. It was found that with 0·125% (vw −1) α-amylase (Termamyl) at 80°C, a 1 h liquefaction was sufficient. At 35°C and an optim...

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Bibliographic Details
Published in:Biomass 1985, Vol.7 (3), p.171-183
Main Authors: Poosaran, N., Heyes, R.H., Rogers, P.L.
Format: Article
Language:English
Subjects:
amylase
cassava
ethanol
fermentation
Saccharomyces
Zymomonas
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Summary:The liquefaction, simultaneous saccharification and fermentation (SSF) of cassava starch (225 g litre −1 glucose equivalent) were investigated in order to optimize the process. It was found that with 0·125% (vw −1) α-amylase (Termamyl) at 80°C, a 1 h liquefaction was sufficient. At 35°C and an optimum AMG concentration of 0·7% (vw −1), Zymomonas mobilis ZM4 had a fermentation time of 20 h, giving 114 g litre −1 of ethanol (95% of the theoretical yield on starch supplied). Under the same conditions, Saccharomyces uvarum ATCC 26602 had a fermentation time of 33 h, producing 106 g litre −1 of ethanol (a 90% yield). The main factors affecting the rate of fermentation with varying AMG concentrations were glucose limitation (at low AMG concentrations) and substrate inhibition (at high AMG concentrations).
ISSN:0144-4565
1878-2523
DOI:10.1016/0144-4565(85)90006-X