α-Amylase and glucoamylase immobilized on chitin and ceramic supports

The enzymes, α-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3), are used in starch liquefaction and saccharification for the production of glucose. A significant percentage of the costs involved in industrial ethanol production from starch is a result of the use of soluble enzymes. If these enzym...

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Bibliographic Details
Published in:Biomass 1987, Vol.13 (1), p.25-32
Main Authors: Xavier, Marcia Tosta, Soares, Valéria Ferreira, Freire, Denise Guimarães, Moreira, Clarissa Perrone, Mendes, Mariza Fonseca, Bon, Elba
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
enzyme immobilization
Food industries
Fundamental and applied biological sciences. Psychology
glucoamylase
Immobilization of enzymes and other molecules
Immobilization techniques
manioc starch hydrolysis
Methods. Procedures. Technologies
Starch and starchy product industries
α-Amylase
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Summary:The enzymes, α-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3), are used in starch liquefaction and saccharification for the production of glucose. A significant percentage of the costs involved in industrial ethanol production from starch is a result of the use of soluble enzymes. If these enzymes were immobilized on solid supports reutilization might be possible, thus reducing costs. This work compares the properties of α-amylase and glucoamylase, immobilized on two different kinds of supports: chitin and ceramic. A comparison was made in order to evaluate which support gave the highest levels of enzymatic activity and stability.
ISSN:0144-4565
1878-2523
DOI:10.1016/0144-4565(87)90069-2