Atypical ϑ-protein kinase c displays a unique developmental expression pattern in rat brain

The expression of atypical ϑ-protein kinase C (PKC) was examined during prenatal and postnatal rat brain development. Immunoblot as well as transcript analysis revealed a dramatic increase in expression at 2–3 days post-birth, which declined thereafter and remained at levels observed in the adult br...

Full description

Saved in:
Bibliographic Details
Published in:Brain research. Developmental brain research 1995-04, Vol.85 (2), p.239-248
Main Authors: Hunter, Starla E., Seibenhener, Michael L., Wooten, Marie W.
Format: Article
Language:English
Subjects:
Development
Isoform
Protein kinase C
Zeta
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The expression of atypical ϑ-protein kinase C (PKC) was examined during prenatal and postnatal rat brain development. Immunoblot as well as transcript analysis revealed a dramatic increase in expression at 2–3 days post-birth, which declined thereafter and remained at levels observed in the adult brain. The expression of ϑ-PKC precedes that of the other PKC isoforms in developing rat brain. Subcellular fractionation of pup and adult brain documented distribution between all three distinct fractions (A,B,C), including the low speed pellet composed of nuclei. In adult brain, the kinase was enriched in the A fraction of the sucrose gradient. Specific substrate proteins of ϑ-PKC were characterized in each of the subccllular fractions from both pup and adult brain. Four predominant proteins pp76, pp60-doublet, pp54 and pp45 were identified as ϑ-PKC endogenous substrates. All four proteins were phosphorylated on serine residues, while the pp60-doublet was also phosphorylated on tyrosine. The pp60-doublet was the most predominant substrate, specifically enriched in the A fraction of a sucrose gradient of adult brain and immunoprecipitated by monoclonal antibody to pp60 c- src .
ISSN:0165-3806
DOI:10.1016/0165-3806(94)00219-P