Double minutes in the papillary thyroid cancer cell line PTC-1113A

The cell line PTC-1113A was established from a metastasizing recurrent papillary thyroid cancer. The cell line was growing as monolayer and showed a complex karyotype with chromosome numbers ranging from 30 to 140/metaphase. A proportion of metaphases contained double minutes and/or pulverized chrom...

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Bibliographic Details
Published in:Cancer genetics and cytogenetics 1996-08, Vol.90 (1), p.70-74
Main Authors: Herrmann, Marille E., Lalley, Peter A., Rydstedt, Linea L., Meese, Eckart, Lang, Charles H., Abumrad, Naji N., Moll, Ute M., Talpos, Gary B.
Format: Article
Language:English
Subjects:
Aged
Biological and medical sciences
Carcinoma, Papillary - genetics
Carcinoma, Papillary - pathology
Chromosomes, Human - ultrastructure
Endocrinopathies
Gene Amplification
Humans
In Situ Hybridization, Fluorescence
Male
Malignant tumors
Medical sciences
Neoplasm Metastasis
Thyroid Neoplasms - genetics
Thyroid Neoplasms - pathology
Thyroid. Thyroid axis (diseases)
Tumor Cells, Cultured
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Summary:The cell line PTC-1113A was established from a metastasizing recurrent papillary thyroid cancer. The cell line was growing as monolayer and showed a complex karyotype with chromosome numbers ranging from 30 to 140/metaphase. A proportion of metaphases contained double minutes and/or pulverized chromosomes. Extrachromosomal DNA seemed to originate from a B-group chromosome. A chromosome 4 painting probe hybridized to extrachromosomal material, representing double minutes (dmin) and possibly minutes. In addition, fluorescence in situ hybridization (FISH) with the chromosome 4 library detected a translocation chromosome and a pulverized chromosome originating from chromosome 4. PTC-1113A is, to our knowledge, the single papillary thyroid cancer cell line demonstrating evidence of gene amplification.
ISSN:0165-4608
1873-4456
DOI:10.1016/0165-4608(96)00048-9