Incidence and toxigenicity of Aeromonas hydrophila in seafood

Three selective media, Oxoid Aeromonas agar (OA), blood ampicillin agar (BA) and starch ampicillin agar (SA) were used to evaluate the presence of Aeromonas hydrophila in 66 samples of oyster, shrimp, fish and surimi products. Oyster had the highest incidence, with 50% positive, whilst no A. hydroph...

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Bibliographic Details
Published in:International journal of food microbiology 1996-08, Vol.31 (1), p.121-131
Main Authors: Tsai, Guo-Jane, Chen, Tzy-Huah
Format: Article
Language:English
Subjects:
AEROMONAS HYDROPHILA
Aeromonas hydrophila - growth & development
Aeromonas hydrophila - isolation & purification
Aeromonas hydrophila - metabolism
Animals
Biological and medical sciences
CREVETTE
Culture Media - chemistry
Cytotoxin
Cytotoxins - metabolism
Decapoda (Crustacea) - microbiology
FISH
Fish and seafood industries
Fishes - microbiology
Food industries
Fundamental and applied biological sciences. Psychology
GAMBAS Y CAMARONES
HAEMOLYSINS
HEMOLISINAS
Hemolysin
Hemolysin Proteins - metabolism
HEMOLYSINE
HUITRE
MORBIDITE
MORBIDITY
MORBOSIDAD
OSTRA
Ostreidae - microbiology
OYSTERS
PESCADO
POISSON (ALIMENT)
PRAWNS AND SHRIMPS
Seafood
SURIMI
Temperature
TOXINAS
TOXINE
TOXINS
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Summary:Three selective media, Oxoid Aeromonas agar (OA), blood ampicillin agar (BA) and starch ampicillin agar (SA) were used to evaluate the presence of Aeromonas hydrophila in 66 samples of oyster, shrimp, fish and surimi products. Oyster had the highest incidence, with 50% positive, whilst no A. hydrophila was found in the surimi. Of the three selective media, BA displayed the highest recovery rate of A. hydrophila from seafood. Forty-eight isolates from this survey were tested for their capability to produce hemolysin and cytotoxin. Hemolysin was produced by 79.2% of the isolates and cytotoxin was produced by 91.7% of the isolates in brain heart infusion broth. One of the toxin-producing isolates from oyster, strain 8–169, was further tested for growth and toxin production in oyster, shrimp and fish at various temperatures. This particular isolate grew best and had highest toxin production in oyster. Hemolysin and cytotoxin were produced earlier at 28 °C than at 37 °C, and titers of hemolysin were also higher at 28 °C. At 5 °C, it was able to grow and produce hemolysin in oyster.
ISSN:0168-1605
1879-3460
DOI:10.1016/0168-1605(96)00972-5