LB film immobilized immunosensor with a charge detection type amplifier

In order to fabricate a miniturized solid-state sensor, a novel potentiometric immunosensor was designed by incorporating a new amplification system of charge detection type and an electrode covered with immunogloblin-G (IgG, antigen) immobilized in a Langmuir-Blodgett (LB) film. A surface potential...

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Bibliographic Details
Published in:Applied surface science 1988-09, Vol.33, p.1332-1338
Main Authors: Katsube, Teruaki, Yaji, Tamaki, Suzuki, Kenji, Kobayashi, Shinichi, Kawaguchi, Takeyuki, Shiro, Takashi
Format: Article
Language:English
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Summary:In order to fabricate a miniturized solid-state sensor, a novel potentiometric immunosensor was designed by incorporating a new amplification system of charge detection type and an electrode covered with immunogloblin-G (IgG, antigen) immobilized in a Langmuir-Blodgett (LB) film. A surface potential change of 1 μV generated by the immuno-reaction between IgG and anti-IgG (antibody) could be measured with the amplification system, which made it possible to detect the anti-IgG with concentrations less than 5 x 10 -8M. The intensity of surface potential change observed in the reaction was successfully correlated with the activity of the immobilized IgG by using luminescent enzyme immunoassay.
ISSN:0169-4332
1873-5584
DOI:10.1016/0169-4332(88)90452-7