Acid secretion and membrane reorganization in single gastric parietal cell in primary culture

• Published in: Biology of the cell 1990, Vol.69 (3), p.223-232
• Main Authors: Mangeat, Paul, Gusdinar, Tutus, Sahuquet, Alain, Hanzel, David K, Forte, John G, Magous, Richard
• Format: Article
• Language: English
• Subjects: acid secretion; Actins - analysis; Adenosine Triphosphatases - metabolism; Aminacrine; Animals; Cell Membrane - ultrastructure; Cells, Cultured; Cytoskeletal Proteins; ezrin; Gastric Acid - secretion; gastric parietal cell; H(+)-K(+)-Exchanging ATPase; Histamine - pharmacology; Image Processing, Computer-Assisted; Intracellular Membranes - ultrastructure; membrane skeleton; Microscopy, Fluorescence; Parietal Cells, Gastric - drug effects; Parietal Cells, Gastric - secretion; Parietal Cells, Gastric - ultrastructure; Phosphoproteins - analysis; Photomicrography; Rabbits; Secretory Rate - drug effects; Stimulation, Chemical; Vacuoles - ultrastructure; videomicroscopy; Videotape Recording
• ISSN: 0248-4900; 1768-322X
• DOI: 10.1016/0248-4900(90)90349-8

A digitally-enhanced videomicroscopy study of rabbit gastric parietal cells in primary culture was performed using alternate observations with differential interference contrast and fluorescence optics of cells mounted and perfused on a temperature-controlled microscope stage. The effect of histamine, a physiological effector of acid secretion, was followed. Isolated parietal cells possess an internal apical vacuole, which kept the cell in a pseudopolarized state. This apical vacuole is a site of acid secretion. This was demonstrated by the direct visualization of the uptake of the fluorescent weak base 9-amino acridine and of the concomitant enormous swelling of the acid vacuole which reached an estimated size of 3–7 times the normal cell volume. This morphological change of shape and acidification of apical vacuoles was fully reversible and cells could respond to successive stimulations. A quantitative study of these events provided a value of the acid accumulation index for each single cell in response to histamine. Individual cell response varied within a factor of 7. The cellular localization of the proton pump complex responsible for acid secretion and of the major components of the secretory microvilli, actin and ezrin, a histamine-dependent phosphorylation target of protein kinase A, were detected by indirect immunofluorescence microscopy in resting and stimulated cells. Both actin and ezrin colocalized at the apical vacuole membrane in resting and stimulated cells, whereas the proton pump shifted from an intracytoplasmic pool to the apical vacuole membrane upon stimulation.