Response of hydrogen peroxide scavenging system in two soybean cultivars exposed to SO2 : experimental evidence for the detoxification of SO2 by enhanced H2O2 scavenging components

• Published in: Environmental pollution (1987) 1993, Vol.82 (1), p.99-105
• Main Authors: RAO, M. V, DUBEY, P. S
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Non agrochemicals pollutants; Phytopathology. Animal pests. Plant and forest protection; Pollution effects and side effects of agrochemicals on crop plants and forest trees. Other anthropogenic factors; Pollution effects. Side effects of agrochemicals
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/0269-7491(93)90167-M

The impact of SO(2) on superoxide dismutase (SOD) and the ascorbate-glutathione cycle was investigated in a tolerant (cv. Punjab-1) and a sensitive (cv. JS 7244) cultivar of soybean (Glycine max (L.) Merr.). In spite of SO(2) stimulated SOD activities in both the cultivars, only cv. JS 7244 has significantly enhanced Malondialdehyde (MDA) contents. This differential response was attributed to the ability of cv. Punjab-1 to enhance glutathione reductase (GR) activity and to maintain high GSH/GSSG and ASA/DHA ratios. Post-fumigation analysis indicated the ability of cv. Punjab-1 to maintain SO(2)-enhanced antioxidants, whilst they declined in cv. JS 7244 the moment fumigation was terminated. Exposure of SO(2)-acclimated plants (cv. Punjab-1) with their enhanced antioxidants to 250 microg m(-3) SO(2) for 6 h exhibited no enhanced cellular injury (MDA content) when compared to that of control plants with their normal antioxidant levels. These results indicate a relation between the ability of a plant to maintain reduced glutathione (GSH) and ascorbate (ASA) and SO(2) tolerance, and they also present evidence for the ability of plants, with elevated antioxidants, to tolerate SO(2)-induced oxygen-free radical toxicity.