An electrochemical bienzyme membrane sensor for free cholesterol

An electrochemical bienzyme membrane sensor for free cholesterol was developed. The membrane poly(tetrafluoroethylene)-g.co-acrylic acid (Teflon-g.co-AA), contains catalase and cholesterol oxidase immobilized covalently and this bienzyme sequence transforms cholesterol and produces oxygen which is d...

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Bibliographic Details
Published in:Bioelectrochemistry and Bioenergetics 1992, Vol.28 (1), p.105-115
Main Authors: Oliveira Brett, Ana Maria C.F., Gil, Maria Helena, Piedade, Ana Paula
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Other biological molecules
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Summary:An electrochemical bienzyme membrane sensor for free cholesterol was developed. The membrane poly(tetrafluoroethylene)-g.co-acrylic acid (Teflon-g.co-AA), contains catalase and cholesterol oxidase immobilized covalently and this bienzyme sequence transforms cholesterol and produces oxygen which is detected at a standard oxygen electrode. Preparation of graft membranes of Teflon was achieved using gamma irradiation at 400 Gy h −1 for different intervals of time. The single membrane containing immobilized catalase and cholesterol oxidase was characterized and the activity and stability of the enzymes studied. This amperometric biosensor for the determination of free cholesterol showed good reproducibility, a linear response for a range of cholesterol concentrations from 90 μM to 0.55 mM and a reasonable lifetime of 1 month.
ISSN:0302-4598
DOI:10.1016/0302-4598(92)80006-3