Pre-irradiated photo-oxidized psoralen induces hemolysis and changes in electrical parameters of erythrocyte membranes

The effects of pre-irradiated photo-oxidized psoralen (POP) on human erythrocytes (RBCs) were investigated. Psoralen in ethanol solutions was photo-oxidized and then added to a suspension of erythrocytes. The hemolysis and the electric impedance of the suspension were measured in the frequency range...

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Bibliographic Details
Published in:Bioelectrochemistry and Bioenergetics 1993, Vol.31 (2), p.193-202
Main Authors: Wunderlich, S., Pliquett, F., Morich, E., Kyagova, A.A., Sukhorukov, V.L., Potapenko, A.Ya
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological effects of radiation
Fundamental and applied biological sciences. Psychology
Ionizing radiations
Tissues, organs and organisms biophysics
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Summary:The effects of pre-irradiated photo-oxidized psoralen (POP) on human erythrocytes (RBCs) were investigated. Psoralen in ethanol solutions was photo-oxidized and then added to a suspension of erythrocytes. The hemolysis and the electric impedance of the suspension were measured in the frequency range 5–20 MHz. The membrane capacity C M, and the extracellular and intracellular conductivities k a and k i were calculated from these data. There appeared to be a threshold concentration of POP for hemolysis. When the concentration of POP slightly exceeded this threshold, the hemolytic effect rapidly increased to a maximum, but a further increase in POP concentration slowed down the rate of hemolysis. POP induced a decrease in extracellular conductivity and an increase in membrane capacity, but did not affect intracellular conductivity. The changes in extracellular conductivity correlated well with hemolysis.
ISSN:0302-4598
DOI:10.1016/0302-4598(93)80007-H