Cryomicroscopy of liquid and semiliquid specimens: Direct imaging versus replication

Direct imaging techniques are compared to freeze-fracture replication as applied to electron microscopic specimens of frozen liquid and semiliquid systems. The processes of cryofixation and sample preparation are discussed as are the problems of contrast and resolution. The pros and cons of the two...

Full description

Saved in:
Bibliographic Details
Published in:Ultramicroscopy 1984, Vol.14 (3), p.211-218
Main Authors: Bachmann, Luis, Talmon, Yeshayahu
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Direct imaging techniques are compared to freeze-fracture replication as applied to electron microscopic specimens of frozen liquid and semiliquid systems. The processes of cryofixation and sample preparation are discussed as are the problems of contrast and resolution. The pros and cons of the two techniques indicate that direct imaging and freeze fracturing are complementary rather than alternative techniques.
ISSN:0304-3991
1879-2723
DOI:10.1016/0304-3991(84)90089-5