Competitive binding of calcium and magnesium to streptococcal lipoteichoic acid

Equilibrium dialysis was used to investigate the binding capacity and affinity of lipoteichoic acid (LTA) from the Gram-positive bacterium Streptococcus sanguis for calcium and magnesium by a competitive method. LTA was shown to bind approx. 1 mol of either calcium or magnesium per mole phosphate. C...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1995-08, Vol.1245 (1), p.94-98
Main Authors: Keith Rose, R., Hogg, Stephen D.
Format: Article
Language:English
Subjects:
Binding, Competitive
Buffers
Calcium
Calcium - metabolism
Dental Plaque - microbiology
Lipopolysaccharides - isolation & purification
Lipopolysaccharides - metabolism
Lipoteichoic acid
Magnesium
Magnesium - metabolism
S. sanguis
Streptococcus sanguis
Teichoic Acids - isolation & purification
Teichoic Acids - metabolism
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Summary:Equilibrium dialysis was used to investigate the binding capacity and affinity of lipoteichoic acid (LTA) from the Gram-positive bacterium Streptococcus sanguis for calcium and magnesium by a competitive method. LTA was shown to bind approx. 1 mol of either calcium or magnesium per mole phosphate. Calcium and magnesium dissociation constants were found to be 8.39 ± 0.31 mmol/l and 15.01 ± 2.01 mmol/l respectively, indicating that S. sanguis LTA will preferentially bind calcium. LTA may act as a calcium buffer by reducing the free calcium concentration to which the cell is exposed. The capacity to produce large quantities of LTA could thus be as important as aciduricity in selection of species at caries-prone sites.
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(95)00073-K